Determination of monocentenylglucoside in grape

2020-02-17 16:56:17

However, due to the lack of chromophore group in alkyl glucoside molecule, UV detector can not be used, differential refractive detector can be used, but the sensitivity is not high and gradient elution is not possible. At present, the evaporative light scattering detector is widely used as a detection method for the analysis of alkyl glucosides, which has high sensitivity and gradient elution.

The results of the determination of trimethyleva derivative and different stationary phases were compared. It was found that the reversed-phase liquid chromatography was more suitable for the analysis of these alkyl glucosides.

In recent years, with the rapid development of liquid chromatography-mass spectrometry, it has been used more and more in the analysis of alkyl glucosides. When it is used with on-line, it can achieve the separation and qualitative analysis of samples at the same time. Zhang Zhengzhu et al. Made liquid chromatography-mass spectrometry (LC-MS) analysis of the alkyl glucoside perfume precursors in the fresh tea leaves, preliminarily separated the alkyl glucoside perfume precursors in the fresh tea leaves, determined the gametophytes and sugars after enzymolysis, analyzed and identified most of the alkyl glucoside perfume precursors in the fresh tea leaves. It is especially suitable for the analysis of alkyl glucosides in complex samples, such as environmental samples, pan washing agents, etc. at this time, selective ion detection or extraction ion detection technology can be used, which can eliminate the interference of accompanying substances and obtain high sensitivity.

The qualitative and quantitative analysis of chromatography is based on the complete separation of the components in the sample, and the application of MS soft ionization technology can directly characterize the complex mixture which is not stable or volatile, and does not need to be separated by the chromatography in danger, which is characterized by fast and high sensitivity. Atmospheric pressure ionization electrospray ionization mass spectrometry can be used directly for qualitative analysis of alkyl glucosides. The components of alkyl glucosides with different chain length and polymerization degree can be detected in the form of excimer ions. In the positive ion detection mode, alkyl glucoside molecules form protonated molecular ions or add ions or group ions with,, and other cations; in the negative ion detection mode, alkyl glucoside molecules mainly form deproton ions.

In addition, matrix assisted laser desorption time of flight mass spectrometry and tandem mass spectrometry are also used to analyze alkyl glucosides. Because of its fast speed and high sensitivity, soft ionization mass spectrometry can be used for the quality control of the production of alkyl glucoside and the study of the degradation mechanism of alkyl glucoside in the environment. These methods can also be used for the analysis of the content of alkyl glucoside in samples.